FlyPredictome Network Viewer
Interactive exploration of the Drosophila protein-protein interaction atlas.
FlyPredictome Database → — search any protein pair for predicted structures, PAE heatmaps, and the iLIS metric.
Network Construction
This network shows evidence-supported positive PPIs from the FlyPredictome atlas:
- Drosophila protein pairs were scored with AlphaFold-Multimer and iLIS
- Positive PPIs were selected at iLIS ≥ 0.223 (10% FPR)
- Only PPIs with prior evidence were retained: fly literature, BioGRID, genetic interaction (MIST), or conserved interolog (DIOPT ≥ 4)
- Proteins with ≥ 2 connections were kept (degree ≥ 2 filter)
- The giant connected component was extracted
Evidence Filter
- All — All evidence types combined
- Fly only — PPIs supported by Drosophila evidence (literature, BioGRID, GI) but not conserved interologs
- Interolog only — Conserved interologs (DIOPT ≥ 4) without fly evidence
- Both — PPIs supported by both fly evidence and conserved interologs — highest confidence
Edge colors: blue = fly evidence, teal = both, orange = interolog only.
FPR Filter
- ≤10% — All evidence-supported PPIs (iLIS ≥ 0.223)
- ≤5% — Higher-confidence PPIs (iLIS ≥ 0.339)
- ≤1% — Highest-confidence PPIs (iLIS ≥ 0.551)
Navigation
- Scroll to zoom · Drag to pan · Double-click to drill down
- Breadcrumb links to go back
Search
- Type gene symbol or FBgn ID — single match auto-jumps to protein view
- ESC to clear
Multi-Protein Analysis
- Shift+Click to select proteins
- Find Connections — shortest paths between selected proteins
- Shared Network — common neighbors
Protein List
- Click Protein List (landing page) or List (header) to paste a list of protein symbols
- Select & Highlight — marks matching proteins on the current view
- Show Subnetwork — extracts a subnetwork with your genes plus connector proteins that link ≥2 of them
Keyboard Shortcuts
- Escape — go up one level or clear search
- / — focus search box
- F — fit/reset zoom
- H — return to home
Links
- FlyPredictome Database — search any protein pair for predicted structures, PAE heatmaps, and the iLIS metric
- AFM-LIS — iLIS analysis pipeline
- LIVIA — interactive 3D structure visualization and interface analysis
- GitHub — source code and data for this viewer
Sharing
Direct links to proteins and clusters are supported via URL hash:
#gene/Akt1 · #cluster/5 · #sub/2/6 · #overview
How to Cite
Kim et al., FlyPredictome: a structural atlas of predicted protein-protein interactions in Drosophila. bioRxiv (2026). doi: 10.64898/2026.04.14.718529
Interactive network viewer: flyark.github.io/FlyPredictome-network